Figure 4.

Circadian rhythm dysfunction (CRD) increased enteric cyanobacteria abundance in SOD1G93A mice. (A) Intestinal bacterial abundance profiles at the level of phylum among the four groups at three time points using 16S high throughput sequencing. Flora were presented in the form of averages. Sample capacity of each group was 4–8. (B) Differences and distances of each sample between the amyotrophic lateral sclerosis (ALS) + CRD group and the ALS group at day 60 and 90, respectively, reflected by Principle Component Analysis (PCA). (C) Comparisons of relative abundances of cyanobacteria among the four groups at different disease stages. N = 4–6 per group per time point, *p < 0.05. Error bars represent SEM.