Figure 1.

Differential effects of dietary LC-PUFAs on the relative cerebral cortex fatty acid content in female mice under different reproductive conditions. Fatty acid (FA) composition of each diet is shown in Table 1. The FAs from brain cortex were obtained and analyzed as described in section Materials and Methods. White bars represent the relative FA content from cerebral cortex of intact animals fed with standard food (SF). Gray and black bars represent the data from animals fed with DI or DII n−6/n−3 LC-PUFA diets, respectively. Vertical axis represents the percentage of variation induced by each diet relative to SF. Data are represented as mean ± SEM of 4 animals per group, except those fed with SF (n = 3). Horizontal axis indicates the different reproductive status: CO, intact, sham-operated; OVX, ovariectomized, placebo-treated; OVX-E, ovariectomized, estradiol-treated. Only some representative p-values for the main effects of diet (D), hormone status (H) and their interaction (D × H) were indicated in each graph and the remaining data were detailed in Table 4.