Table 5.
Linear contrasts of the differential effects of dietary n−6/n−3 ratio on cerebral cortex lipid content under different reproductive conditions.
| Lipid class | Main effects | p-value | 95% CI |
|---|---|---|---|
| A. Total Ceramides | DI-SF (CO) | 0.0001 | 230.1, 578.3 |
| DII-SF (CO) | 0.018 | 41.3, 389.5 | |
| DI-DII (CO) | 0.024 | 27.6, 350.0 | |
| DI-DII (OVX) | 0.707 | −190.6, 13.8 | |
| DI-DII (OVX–E) | 0.007 | 78.8, 427.1 | |
| DI-DII (CO+OVX–E) | 0.001 | 102.2, 339.5 | |
| [DI-DII (CO+OVX–E)]-[DI-DII (OVX)] | 0.017 | 50.1, 450.4 | |
| B. dh-Ceramides | DI-SF (CO) | >0.00001 | 551.9, 1236.4 |
| DII-SF (CO) | 0.014 | 99.3, 783.8 | |
| DI-DII (CO) | 0.008 | 135.7, 769.5 | |
| DI-DII (OVX) | 0.577 | −402.9, 230.8 | |
| DI-DII (OVX–E) | 0.013 | 104.6, 789.1 | |
| DI-DII (CO+OVX–E) | 0.001 | 216.5, 682.9 | |
| [DI-DII (CO+OVX–E)]-[DI-DII (OVX)] | 0.010 | 142.3, 929.2 | |
| C. Total Sphingomyelins | DI-SF (CO) | >0.00001 | 500.9, 1082.9 |
| DII-SF (CO) | 0.013 | 88.0, 669.9 | |
| DI-DII (CO) | 0.005 | 143.5, 682.3 | |
| DI-DII (OVX) | 0.512 | −355.5, 183.3 | |
| DI-DII (OVX–E) | 0.023 | 54.4, 636.4 | |
| DI-DII (CO+OVX–E) | 0.001 | 180.9, 577.4 | |
| [DI-DII (CO+OVX–E)] - [DI-DII (OVX)] | 0.009 | 130.7, 799.7 | |
| E. MHC | DI-SF (CO) | >0.00001 | 432.0, 948.6 |
| DII-SF (CO) | 0.003 | 165.7, 682.3 | |
| DI-DII (CO) | 0.031 | 27.2, 505.4 | |
| DI-DII (OVX) | 0.323 | −355.1, 123.2 | |
| DI-DII (OVX–E) | 0.067 | −18.9, 497.7 | |
| DI-DII (CO+OVX–E) | 0.007 | 76.8, 428.8 | |
| [DI-DII (CO+OVX–E)] - [DI-DII (OVX)] | 0.018 | 71.8, 665.7 | |
| E. MHC | DI-SF (CO) | >0.00001 | 346.9, 734.7 |
| DII-SF (CO) | 0.003 | 126.8, 514.6 | |
| DI-DII (CO) | 0.019 | 40.6, 399.6 | |
| DI-DII (OVX) | 0.589 | −226.6, 132.4 | |
| DI-DII (OVX–E) | 0.041 | 8.8, 396.6 | |
| DI-DII (CO+OVX–E) | 0.003 | 79.3, 343.5 | |
| [DI-DII (CO+OVX–E)] - [DI-DII (OVX)] | 0.025 | 35.6, 481.4 | |
| F. C18 GSO3 | DI-SF (CO) | 0.0001 | 519.4, 1343.0 |
| DII-SF (CO) | 0.019 | 93.1, 916.6 | |
| DI-DII (CO) | 0.030 | 45.1, 807.6 | |
| DI-DII (OVX) | 0.934 | −366.0, 396.5 | |
| DI-DII (OVX–E) | 0.001 | 321.0, 1083.5 | |
| DI-DII (CO+OVX–E) | 0.0001 | 294.7, 833.9 | |
| [DI-DII (CO+OVX–E)] - [DI-DII (OVX)] | 0.024 | 82.1, 1016.0 |
DI: High dietary n−6/n−3 ratio; DII: Low n−6/n−3 ratio; SF: Standard food. CO: Intact control female mice; OVX: Ovariectomized, placebo-treated female mice; OVX–E: Ovariectomized, estradiol-treated female mice. Data represented in Figure 2 were analyzed by 2 × 3 factorial ANOVA. In cases where the influence of reproductive status on diet action was absent and significant interaction between the factors was not detected, data from those animals were grouped to emphasize the main dietary effects. The most significant p-values and 95% confidence intervals are highlighted.