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. 2018 Apr 30;9:1739. doi: 10.1038/s41467-018-03590-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — C/EBPβ modulates H3K79 methylation to reprogram gene expression. a Meta-analysis of the averaged H3K79me2/me3 ChIP-seq signals of genes across ±10 kb genomic regions flanking the TSS. b Venn diagrams showing the overlap of genes with decreased H3K79me2/me3 (C13^*-shCEBPB vs. C13^*-shcontrol) and C/EBPβ-targeted genes (Pearson’s chi-squared test). c Normalized C/EBPβ and H3K79me2/me3 ChIP-seq signals for the representative genes (EREG and SLC38A1). d Supervised clustering of genes that were consistently differentially expressed (P < 0.05) upon C/EBPβ knockdown in C13^* and SKOV3 cells. e, f IPA of the differentially expressed genes (e) and KEGG pathway analysis of downregulated genes (f) upon C/EBPβ knockdown in C13^* and SKOV3 cells. Arrows indicate P = 0.05. Negative z-scores (blue) represent the suppressed state of the function. g Scatter plot showing genome-wide changes in the values of C/EBPβ binding (x-axis), gene expression (y-axis), and averaged H3K79me2/me3 (colored) in C13^*-shCEBPB vs. C13^*-shcontrol. Positive and negative values reflect increases and decreases, respectively. h Analysis of C/EBPβ ChIP-qPCR (pink), H3K79me2/me3 ChIP-qPCR (blue), and RT-qPCR (purple) in C13^* cells. Gene names in red indicate documented cisplatin-resistance genes in ovarian cancer. *P < 0.05; n.s. not significant