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. 2018 Apr 30;9:1739. doi: 10.1038/s41467-018-03590-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — C/EBPβ recruits the methyltransferase DOT1L to target genes that methylate H3K79. a Venn diagrams showing the overlap of C/EBPβ- and DOT1L-targeted genes (chi-squared test). b Correlation analysis of C/EBPβ binding sites and DOT1L binding sites. Red dots represent genes with two peak centers located less than 2500 bp apart. c Representative results of C/EBPβ and DOT1L ChIP-qPCR. Primers were chosen according to the ChIP-seq results (upper panel). d C/EBPβ interacts with DOT1L. Lysates from C13^* cells were immunoprecipitated (IP) with a mouse anti-C/EBPβ antibody and analyzed by western blotting using a rabbit anti-DOT1L antibody (upper panel) or the reciprocal (lower panel). e ChIP-reChIP experiments with anti-C/EBPβ and anti-DOT1L antibodies. Mouse IgG (mIgG) and rabbit IgG (rIgG) were used as negative controls. f Meta-analysis of the averaged DOT1L ChIP-seq signal of genes across a ±10 kb genomic region flanking the TSS. g Normalized C/EBPβ and DOT1L ChIP-seq signal of the representative C/EBPβ-DOT1L co-targeted genes (EREG and SLC38A1). h Analysis of DOT1L ChIP-qPCR (green), H3K79me2/me3 ChIP-qPCR (blue), and RT-qPCR (purple) in C13^* cells. Gene names in red indicate documented cisplatin-resistance genes in ovarian cancer. i The protein levels of C/EBPβ-DOT1L co-targeted genes in the indicated OV2008 cells were detected by western blotting. Uncropped images of blots are shown in Supplementary Figure 25. *P < 0.05; ***P < 0.001