Figure 3.

Successive decolorization of Indigo Carmine by IOX. The reaction mixture contained 10 mU mL⁻¹ of IOX, 0.15 mM Indigo Carmine, 0.1 mM iodide, and 20 mM sodium acetate buffer (pH 5.5). After complete decolorization, 0.15 mM of Indigo Carmine was sequentially added to the reaction mixture a total of 20 times. The molar absorption coefficients of Indigo Carmine were determined in 20 mM sodium acetate buffer (pH 5.5), and 13.3 mM⁻¹·cm⁻¹ was used for quantification.