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. 2018 Apr 30;8:6748. doi: 10.1038/s41598-018-25137-w

Figure 2.

Figure 2

OARD1 knock-out does not affect cell proliferation of HeLa cells. (a) TARG1 expression was analyzed in whole cell lysates of HeLa cells (WT) and the HeLa OARD1−/− clones 3 and 12 by Western blotting using the TARG1-specific monoclonal antibody 3A5. α-Tubulin was detected as loading control. (b) Cell proliferation of HeLa cells and HeLa OARD1−/− clones 3 and 12 was measured by counting living cells over 7 days. (c) HeLa OARD1−/− (cl. 3) cells were stably transduced using a lentiviral vector allowing for doxycycline-inducible re-expression of HA-TARG1 or HA-TARG1-G43E. TARG1 protein expression was analyzed in HeLa OARD1−/− HA-TARG1 wildtype or -G43E cells that were treated with 100 ng/ml doxycycline or were left untreated for 48 h by Western blotting. α-Tubulin levels were detected as a loading control. (d) Cell proliferation of HeLa OARD1−/− HA-TARG1 or HA-TARG1-G43E cells, which were treated with 100 ng/ml doxycycline every 48–72 h or were left untreated (±Dox), was measured over 7 days by counting living cells. (a and c) Full-length blots are presented in Supplementary Fig. S8.