Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 30;8:6748. doi: 10.1038/s41598-018-25137-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

EGFP-TARG1 localizes to transcriptionally active nucleoli independent of ADP-ribosylation. (a) Live cell imaging of U2OS cells transiently expressing EGFP-TARG1 or EGFP together with mCherry-H2B. Intensity profiles for EGFP and mCherry fluorescence signals are displayed that were measured along the arrows depicted in the merge pictures. DIC: differential interference contrast. (b) Live cell imaging of U2OS cells transiently expressing EGFP-TARG1 wildtype (WT), G43E, I44E or G123E together with mCherry-H2B. For intensity profiles, see Supplementary Figures S7. (c) U2OS cells transiently expressing EGFP-TARG1 or EGFP-TARG1-G123E together with mCherry-H2B were treated with 10 ng/ml actinomycin D (ActD) or DMSO for 90 min. Subcellular localization of EGFP-TARG1 (wildtype or G123E) was analyzed by confocal microscopy in living cells.