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Localization and assembly of imported plastid E2. A, Localization of imported and processed plastid E2. After import, chloroplasts were reisolated and fractionated to obtain envelope, thylakoid membrane, and stromal fractions. The precursor and mature forms of E2 are indicated. B, Immunoprecipitation of imported plastid E2. After import, a stromal fraction was prepared and immunoprecipitated with preimmune serum or anti-plastid E1α antibodies.
