Figure 6.

Sequence comparison of six E2 subunits. The first sequence shown is the Arabidopsis plastid PDC E2 sequence (A. t. PLASTID) reported here. Shading of amino acid residues denotes identity between at least two sequences. Hyphens denote alignment gaps in the sequences. Three conserved domains are denoted by their corresponding letters and are overlined after their designation. L, Lipoyl domain; B, subunit-binding domain; I, inner catalytic domain. The “arrow +1” indicates the proposed mature start of the Arabidopsis plastid protein. ▿, Position of the conserved Lys involved in lipoic acid binding. ▾, The only Cys residue in the plastid E2 sequence. The conserved motif of the active site of acetyltransferases is double overlined. The accession numbers of the sequences are as follows: Synechocystis sp., D90915; Arabidopsis (mitochondrial), Z46230; H. sapiens, J03866; S. cerevisiae, J04096; and B. subtilis, M57435.