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Summary of experimental design and analysis. The overall design of the project and subsequent analyses is shown. We performed a series of immunizations with 9 different antigens and included 75 different OmniFlic animals. We harvested 2 lymph nodes from each animal for a total of 150 different samples. Our analysis was focused on three areas: VDJ clonotype diversity by NGS, somatic hypermutation by NGS, and antigen binding by ELISA.
