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. 2018 Apr 24;9:763. doi: 10.3389/fmicb.2018.00763

FIGURE 2.

FIGURE 2

Accumulation of GspR is independent of SmelC776 expression. (A) Expression profiling of GspR. Northern blot detection of GspR abundance in the S. meliloti Rm2011 wild type under different growth and stress conditions in minimal and TY-rich media in exponential and stationary growth phase and in TY-rich medium at 20°C, cold stress; 42°C, heat stress; NaCl, 0.4 mM sodium chloride (osmotic stress); H2O2, 10 mM hydrogen peroxide (oxidative stress); pH 5.6, 20 mM MES (acidic stress); -O2, microoxic conditions. Exposure times were optimized for each panel. 5S rRNA probing was used as RNA loading control. (B–D) Northern blot detection of GspR and SmelC776 RNA variants in Sm2B2019DD or Sm2B2019 strains carrying either pSKControl+ (Control+), pSKSmelC776+ (SmelC776+), or pSKGspR+ (GspR+), 4 h after induction with IPTG.