FIGURE 5.

GspR SL1 represses ctrA post-transcriptionally. (A) Predicted duplexes between GspR and ctrA mRNA. The nucleotide exchanges in the predicted interaction regions of ctrA (ctrA-BS.2 and BS.4) and GspR (GspR 1.1, 1.2, 1.4, and 3.4) mRNAs are indicated in bold. (B) Schematic representations of the genomic regions and the fragments translationally fused to egfp. (C–G) Means of relative fluorescence intensity values of Sm2B2019DD co-transformed with overexpression plasmids carrying control SmelC812, gspR, or its mutant variants and the indicated ctrA translational fusion. Fluorescence measurements have been performed as described in Figure 4.