Fig. 3.

CsPLCPs accumulate during SA treatment and infection. a Abundance of PLCP genes was determined by quantitative RT-PCR after SA treatment. One-year-old Navel oranges (C. sinensis) were sprayed with 2 mM SA or water. Leaf samples were collected after 48 h for RNA extraction and PCR analyses. Cytochrome oxidase subunit 1 (COX, KF933043.1) was used as the internal standard. Graph shows mean ± standard error of three replicates. Asterisks (*) indicate statistically significant differences based on the two-tailed Student’s t-test. p < 0.05 = *, p < 0.01 = **. b Protein abundance of PLCPs was determined in healthy (−) or CLas-infected (+) citrus branches using an anti-AALP antibody. Freshly cut stems were stamped onto nitrocellulose membranes and PLCPs and SDE1 were detected using western blotting. The titer of CLas in each sample was evaluated by quantitative PCR with observed Ct values of 27.97 for symptomatic tissue (+S), and not detected for asymptomatic tissue from the same infected tree (+AS) or tissue from an uninfected tree (−). Ponceau-stained membrane was shown as a control