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. 2018 Apr 30;9:1718. doi: 10.1038/s41467-018-04140-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — SDE1 does not inhibit Solanaceous RCR3 activity. a SDE1 did not elicit cell death in tomato (Moneymaker, Solanum lycopersicum) leaves expressing the immune receptor Cf-2. Purified recombinant proteins FLAG-Avr2-6XHIS or FLAG-6XHIS-SDE1 were infiltrated in tomato leaves in three different concentrations: 1 μg, 100 ng, and 10 ng. Avr2 inhibits the protease activity of RCR3^pim (RCR3 from Solanum pimpinellifolium, which is required for Avr2-triggered cell death in tomato plants expressing Cf-2 produced by S. pimpinellifolium (left). Cf-0 (right) and Cf-2 rcr3 (middle, containing a rcr3 mutant with a premature stop codon) tomatoes were used as negative controls. Picture was taken after 7 dpi. b SDE1 does not inhibit the activity of RCR3^pim. Full length of RCR3^pim-HIS was transiently expressed in N. benthamiana and secreted into the apoplast. Apoplastic fluid was extracted and the active protease was labeled via ABPP in the presence of 0.8, 1.6 or 3.2 μM of purified SDE1 protein. Coomassie brilliant blue (CBB) served as a loading control