Table 7.
The effect of threshold of minimum number of heterozygous genotypes (Het) among the 11 doubled haploid (DH) lines on the number of putative PSVs and MSVs identified and the average observed heterozygosity in those loci among the 50 outbred (non-DH) rainbow trout sampled in this study.
| No. of het genotypes | No. of putative PSVs/MSVs | Average heterozygosity* | Deviation from HW (%)† |
|---|---|---|---|
| Het > 0 | 2,767,612 | 0.35 | 28 |
| Het > 1 | 1,733,481 | 0.44 | 39 |
| Het > 2 | 1,187,715 | 0.51 | 49 |
| Het > 3 | 836,278 | 0.57 | 58 |
| Het > 4 | 596,329 | 0.62 | 67 |
| Het > 5 | 429,862 | 0.66 | 75 |
| Het > 6 | 311,629 | 0.71 | 81 |
| Het > 7 | 224,252 | 0.75 | 85 |
| Het > 8 | 155,617 | 0.78 | 89 |
| Het > 9 | 97,433 | 0.82 | 92 |
| Het = 11 | 45,757 | 0.85 | 95 |
High rate of observed heterozygosity indicates high occurrence of MSVs and PSVs.
*
Average observed heterozygosity per locus.
†
Percent of loci that deviated from expected Hardy-Weinberg equilibrium (P < 0.05).