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. 2018 Apr 24;12:33. doi: 10.3389/fncir.2018.00033

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Copyright © 2018 Li, Wang, Yao, Liang, Liao, Yang, Zhang, Yan, Jia, Chen and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dendritic Ca²⁺ imaging using electroporation in the Au1 of behaving mice. (A) Schematic of electroporation in the Au1. (B) Reconstruction of an electroporated neuron in the Au1, labeled with OGB-1. (C) Spontaneous Ca²⁺ transients from 5 dendritic shafts (outlined in left part of B). Local Ca²⁺ transients are marked in red. (D) Dendritic Ca²⁺imaging from a region of the neuron during behavior (outlined in right part of B). Average Ca²⁺ transients related to the BBN are marked in red.