FIGURE 1.
Synchrotron excitation deep ultraviolet (DUV) fluorescence imaging of bacterial cells after Titanium dioxide (TiO2) exposure. (A) Fluorescence emission spectra (λexc = 270 nm) of TiO2 320 μg/mL food-grade E171 (in orange) vs. TiO2-NPs P25 (in green) in aggregated (dark color) and dispersed (light color) forms; (B) Overlaid fluorescence images of (a,c) growing Lactococcus lactis IBB477 cells and (b,d) growing Escherichia coli K12 MG1655 cells (in red) exposed to (a,b) P25-D and (c,d) E171-A (in green) at 320 μg/mL. Yellow color indicates TiO2 particles that are co-localized with bacteria. Scale bar corresponds to 5 μm.