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. 2018 Apr 24;9:394. doi: 10.3389/fphar.2018.00394

FIGURE 2.

FIGURE 2

Morphological, histopathological and fibrotic changes in the lungs of mice post bleomycin injury. (A) Representative images of axial slices of reconstructed anatomical X-ray micro-CT images obtained at 21 days post treatment. Bleomycin treated mice show parenchymal opacity in the right lung. (B) Three-dimensional surface-rendered images of the lungs showing changes in the lung density between different treatment groups. (C) Representative images showing gross lung morphology of mice. (D) Representative photomicrographs of hematoxylin and eosin stained lung sections. Scale bar: 100 μm. Bleomycin treated mice developed lungs that had thickened alveolar septae (arrowhead) collapsed alveolar spaces (#) and infiltration of a large number of inflammatory cells (∗) in the peribroncheolar region. (E) Representative images of Masson’s trichrome stained lung sections at 21 days post treatment. Scale bar = 100 μm. (F) Assessment of pulmonary injury by Ashcroft score. Data represents mean ± SEM; n = 4 per group. (G) Hydroxyproline content, an indicator of collagen deposition, at 21 days post treatment. Data represents mean ± SEM of two independent experiments with n = 3–5 per group. ∗p < 0.05; ∗∗p < 0.01;∗∗∗p < 0.001.