Table 2.
Results of Tm values achieved by SSR-HRM analysis from control genomic DNA of five Glycyrrhiza species.
| SSR primers | Melting curve Tm values (°C) ± SD |
||||
|---|---|---|---|---|---|
| G. uralensis | G. glabra | G. inflate | G. eurycarpa | G. pallidiflora | |
| GM-S1 | 75.42 ± 0.05 | 75.23 ± 0.12 | 75.67 ± 0.05 | 76.08 ± 0.08 | 76.04 ± 0.05 |
| GM-S2 | 78.81 ± 0.08 | 78.53 ± 0.10 | 78.11 ± 0.05 | 78.15 ± 0.06 | 79.42 ± 0.05 |
| GM-S3 | 83.13 ± 0.05 | 82.95 ± 0.07 | 83.25 ± 0.05 | 83.29 ± 0.06 | 83.40 ± 0.04 |
| GM-S4 | 81.70 ± 0.06 | 80.18 ± 0.10 | / | 80.84 ± 0.05 | 81.61 ± 0.05 |
| GM-S5 | 80.55 ± 0.10 | 80.36 ± 0.07 | 80.73 ± 0.06 | 80.67 ± 0.06 | 80.63 ± 0.05 |
| GM-S6 | 78.67 ± 0.04 | 78.91 ± 0.08 | 78.64 ± 0.04 | 78.69 ± 0.04 | 76.49 ± 0.08 |
| GM-S7 | 80.23 ± 0.05 | 81.01 ± 0.06 | 81.14 ± 0.05 | 81.10 ± 0.06 | 79.18 ± 0.07 |
| GM-S8 | 79.87 ± 0.10 | 79.59 ± 0.10 | 80.46 ± 0.10 | 80.54 ± 0.06 | 79.15 ± 0.08 |
| GM-S9 | 80.89 ± 0.06 | 81.66 ± 0.08 | 81.51 ± 0.04 | 81.37 ± 0.08 | 82.97 ± 0.05 |