Fig. 2. The effects of nobiletin on basal ΔΨ_m and cell viability against glutamate toxicity in primary cortical neurons.

(A, D) Recording traces of ΔΨ_m using real-time imaging-based fluorometry with TMRE (see ‘METHODS’ for the detailed description). Various concentrations of nobiletin and CPE were superfused over primary cortical neurons on a cover slip in a recording chamber from the arrow point. TMRE fluorescence values from individual cells were normalized to values before drug treatment shown as an arrow. (B, E) Quantification of ΔΨ_m at the end of experiment for panel A and D. (C, F) Effects of nobiletin and CPE on cell viability against glutamate toxicity (100 µM, 20 min) were investigated using MTT assay. Values are the mean±S.E.M. ^*p<0.05, ^**p<0.01, ^***p<0.001 as compared with the control group and ^#p<0.05 as compared with glutamate alone-treated group.