Fig. 3. The effects of nobiletin on glutamate-induced overload of cytosol and mitochondrial calcium in primary cortical neurons.

(A, C) Dual real-time imaging-based fluorometry of [Ca²⁺]c and [Ca²⁺]m were simultaneously conducted in the same neurons (see ‘METHODS’ for the detailed description). Fura-2 and Rohd-2 fluorescence values from individual cells were normalized to values before drug treatment. (B, D) Quantification of Fura-2 and Rohd-2 fluorescence values at the end of experiment for panel A and C. Values are the mean±S.E.M. ^*p<0.05, ^**p<0.01 as compared with the control group and ^#p<0.05 as compared with glutamate alone-treated group. N.S., not statistically significant.