Fig. 5. The effects of K⁺ influx on nobiletin-induced partial mitochondrial depolarization in isolated brain mitochondria.

(A) The effect of FCCP on ΔΨ_m was investigated with TMRE in an isolated brain mitochondrial model (see ‘METHODS’ for the detailed description) using a fluorescence microplate reader, as a positive control. (B, C). The effect of nobiletin on ΔΨ_m was measured with TMRE in the presence or absence of K+ in the medium using a fluorescence microplate reader in an isolated brain mitochondrial model. To remove K+ in the medium, KCl (100 mM) was replaced to CsCl (100 mM). Dose responses 10 min after nobiletin treatment (B) and time courses (C) were analyzed. Values are the mean±S.E.M. ^*p<0.05, ^**p<0.01, ^***p<0.001 as compared with the control group.