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. 2018 Apr 14;12(5):690–704. doi: 10.1002/1878-0261.12192

Figure 1.

Figure 1

PIM2 interacts with TTP. (A, B) HEK293T cells were co‐transfected with HA‐tagged TTP and Flag‐tagged PIM2. After 24‐h culture, cell lysates were prepared in IP buffer and HA‐tagged proteins were immunoprecipitated with anti‐HA antibody (A) or anti‐Flag antibody (B) (IgG as control). The presence of Flag‐ or HA‐tagged proteins was examined by western blotting using anti‐HA or anti‐Flag antibody. (C, D) Co‐IP of endogenous PIM2 and TTP from MCF‐7 cells using anti‐TTP (C) or PIM2 (D) as the antibodies (IgG as a negative control). The presence of PIM2 or TTP proteins in IP elutes was examined by western blotting using anti‐PIM2 or anti‐TTP antibody. (E) Recombinant GST‐tagged TTP or GST alone proteins were incubated with recombinant His‐tagged PIM2 overnight. Anti‐His antibody was used to detect His‐tagged PIM2 proteins in elutes. (F) Confocal immunofluorescence microscopy was performed to analyze the location of TTP and PIM2 in MCF‐7 cells.