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. 2018 Apr 14;12(5):690–704. doi: 10.1002/1878-0261.12192

Figure 6.

Figure 6

PIM2 regulates TTP‐mediated cell proliferation and migration in breast cancer cells. (A) MCF‐7 or MDA‐MB231 cells were transfected with Flag‐tagged PIM2, HA‐tagged TTP or both HA‐tagged TTP and Flag‐tagged PIM2 (empty vector as control). One day after transfection, cells were re‐plated and analyzed for cell growth by counting cell numbers at the indicated time points. (B) MCF‐7 or MDA‐MB231 cells were transfected with Flag‐tagged PIM2, HA‐tagged TTP or both (empty vector as control). One day after transfection, cells were re‐plated with agarose gel to perform colony formation assays. (C) MCF‐7 or MDA‐MB231 cells were transfected with either Flag‐tagged PIM2 or HA‐tagged TTP, or both (empty vector as control). One day after transfection, cells were re‐plated to perform scratch test assays. Cell migration was determined after 1 day. Representative images (100×). (D) MCF‐7 or MDA‐MB231 cells were transfected with either Flag‐tagged PIM2 or HA‐tagged TTP, or both (empty vector as control). One day after transfection, cells were re‐plated to perform transwell assays. Cell numbers were counted for the analysis of cell migration after 12 h. Representative images (100×). All data are mean ± SD of three independent experiments, *P < 0.05.