Skip to main content
. 2018 Mar 30;12(5):579–593. doi: 10.1002/1878-0261.12163

Figure 4.

Figure 4

2‐FF treatment on CF1_T cells induces lower expression of growth factors and reduced cell proliferation rate. (A) Effect of 2‐FF on CF1_T cell proliferation. CF1_T cells were treated, or not, with 2‐FF, for a total of 14 days. At day 5 of treatment, the cells were stained with CFSE dye and then analyzed after another 9 days of treatment (total of 14 days). CFSE dilution was analyzed by flow cytometry and the proliferation index calculated as the fold expansion of the overall culture using the modfit software [n = 8; P < 0.05 (*)]. (B) The gene expression of growth factors and cytokines on 2‐FF‐treated CF1_T cell line was compared to nontreated CF1_T cell line (ratio 1). RQ indicates the relative change in mRNA expression levels from the 2‐FF‐treated cells relative to the untreated cells [n = 6; P < 0.05 (*), P < 0.01 (**), and P < 0.005 (***)].