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. 2018 Mar 30;12(5):579–593. doi: 10.1002/1878-0261.12163

Figure 5.

Figure 5

ERK1/2 and p38 MAPK pathways are downregulated by 2‐FF treatment of CF1_T cell line. (A,B) CF1_T cell line treated with 2‐FF for 7 and 14 days was analyzed regarding their expression of P‐ERK, total ERK1/2 (ERK), P‐p38, and total p38 (p38) kinases by flow cytometry. Ratios between P‐ERK and ERK expression ((P‐ERK/ERK) × 100) and P‐p38 and p38 expression ((P‐p38/p38) × 100) are represented in the graphs A and B, respectively [n = 4; P < 0.05 (*), P < 0.01 (**)]. (C,D) CF1_T cell line was treated with 2‐FF inhibitor for 14 days. Total lysate proteins were stained with anti‐P‐ERK1/2, anti‐ERK1/2, anti‐P‐p38, or anti‐p38 mAb and analyzed by western blot. Βeta‐tubulin expression was analyzed as a loading control. Band intensity was measured in imagej software, and P‐ERK/ERK ratio (C) and P‐p38/p38 ratio (D), after normalization with loading control, are represented in the graphs (n = 4).