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. 2018 Apr 6;12(5):630–647. doi: 10.1002/1878-0261.12186

Figure 6.

Figure 6

Sensitivity of CML CD34+ cells to SFK/MEK/IKK inhibition. (A) Number of colonies formed in the absence or presence of the combination of dasatinib and U0126, or the combination of dasatinib, U0126, and PS‐1145. Colonies derived from burst‐forming units erythroid (BFU‐E), multilineage granulopoietic, erythroid, macrophage, and megakaryocytic colony‐forming units (CFU‐GEMM), granulocyte–macrophage colony‐forming units (CFU‐GM), and macrophage colony‐forming units (CFU‐M) were scored in situ after 14 days of incubation of CML CD34+ cells in methylcellulose‐based medium with cytokines in the presence or absence of the aforementioned combinations of inhibitors. This experiment was performed using CML CD34+ cells isolated prior to TKI exposure from the bone marrow of three CML patients. Data are shown as the mean and 1 SD. **P < 0.01. (B) RT‐PCR determination of MAP3K8 transcript levels in CML CD34+ cells cultured without inhibitors or with a combination of 50 nm dasatinib, 5 μm U0126, and 10 μm PS‐1145 (D/U/P) or with IM for 7 days. Normalized expression relative to ACTB and S18 rRNA expressed as fold‐change is presented. Samples were cultured and analyzed in triplicate. Results are shown as the mean and 1 SD. *P < 0.05.