Table 2.
Plasmids used in this study.
| Plasmid | Features | Source/reference |
|---|---|---|
| pET24-d | IPTG-inducible T7 promoter, kanamycineR (50 μg/mL), C-terminal His6-tag | Novagen |
| pFBD | pET24d expressing FMN-binding domain of PceC (aa 41–200) | This study |
| pFBD-T168V | pFBD with single mutation expressing a valine variant of Thr168 | This study |
| pETDuet-1 | IPTG-inducible T7 promoter, ampicillineR, two multiple cloning sites, MCS1 with C-terminal His6-tag, MCS2 with C-terminal S∙tagTM | Novagen |
| pFTP1 | pETDuet-1 expressing Ftp1 from MCS2 | This study |
| pFTP2 | pETDuet-1 expressing Ftp2 from MCS2 | This study |