Table 3.

Oligonucleotides used in this study.

Primer name	5′–3′ sequence	Features
FBD-24-F	GCGCCCATGGGACAATCGGTTGATTACAAGGGAATC	NcoI site
FBD-24-R	GCGCCTCGAGTAAATCGTAAGGGTTGGCCCATTG	XhoI site
FBD-T168V-F	ACGGTAACAGGTTCAGTAGTGTCGTCACATGCT	Thr-Val
FBD-T168V-R	AGCATGTGACGACACTACTGAACCTGTTACCGT	Thr-Val
pET24-F2	GGTGATGTCGGCGATATAGG	Sequencing
pET24-R2	CGTTTAGAGGCCCCAAGG	Sequencing
FTP1-F	GCGCCATATGAATGGGAAACCTGTACAACAG	NdeI
FTP1-R	GCGCCTCGAGATCTTTGACGAATTCGTACTC	XhoI
FTP2-F	GCGCCATATGTTGTCTGCAGAGACCAAGG	NdeI
FTP2-R	GCGCCTCGAGTTTGCTTTCTGGGGAAGGTGTC	XhoI
Duet-MCS2-F	TTGTACACGGCCGCATAATC	Sequencing
Duet-MCS2-R	GCTAGTTATTGCTCAGCGG	Sequencing