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. 2018 Mar 13;9(5):589–597. doi: 10.1111/1759-7714.12623

Figure 3.

Figure 3

Overexpression of linc01433 promoted non‐small cell lung cancer (NSCLC) cell proliferation, migration, invasion, and epithelial‐to‐mesenchymal transition (EMT) in vitro. (a) Relative linc01433 expression in H520, H1299, and PC9 cells transfected with pLVX‐EF1α‐IRES‐Puro‐linc01433 vector. Empty vector, OElinc01433. (b, c) The effects of linc01433 overexpression on cell proliferation of H520 and H1299 in an in vitro MTS assay. Transfection of linc01433 significantly promoted the growth of OElinc01433 cells. Empty vector, OElinc01433. (d, e) The effects of linc01433 overexpression on cell migration and invasion. Significantly more H520‐OElinc01433 cells migrated and invaded through the basement membrane compared to empty vector cells. Empty vector, OElinc01433. (f) The effect of linc01433 overexpression on the expression of EMT‐related proteins in H520 cells. Claudin‐1, E‐cadherin, vimentin, β‐catenin, ZEB1, and β‐actin expression determined by Western blotting. Reductions of E‐cadherin and claudin‐1 and increases of vimentin, β‐catenin, and ZEB1 in H520‐OE linc01433 cells. **P < 0.01, ***P < 0.001, ****P < 0.0001.