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. 2018 Mar 13;9(5):589–597. doi: 10.1111/1759-7714.12623

Figure 4.

Figure 4

Knockdown of linc01433 suppressed non‐small cell lung cancer (NSCLC) cell proliferation, migration, invasion, and epithelial‐to‐mesenchymal transition (EMT) in vitro. (a) Relative linc01433 expression in A549 cells transfected with shRNAs. sh‐control, sh‐1#, sh‐2#, sh‐3#. (b) The effect of linc01433 knockdown on cell proliferation of A549 in an in vitro MTS assay. Knockdown of linc01433 significantly suppressed the growth of A549 cells. sh‐control, sh‐2#, sh‐3. (c,d) The effects of linc01433 knockdown on cell migration and invasion. Significantly fewer A549‐ShRNA cells migrated and invaded through the basement membrane compared to A549‐control cells. sh‐control, sh‐2#, sh‐3# (e) The effect of linc01433 knockdown on the expression of EMT‐related proteins in A549 cells. Claudin‐1, E‐cadherin, vimentin, β‐catenin, ZEB1, and β‐actin expression determined by Western blotting. Increases of E‐cadherin and claudin‐1 expression was increased, while vimentin, β‐catenin, and ZEB1 was reduced in A549‐shRNA cells. **P < 0.01, ***P < 0.001, ****P < 0.0001.