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. 2018 Apr 24;9:401. doi: 10.3389/fphar.2018.00401

Figure 3.

Figure 3

Effects of F. x intermedia flower and leaf extracts (FFE and FLE) on the attachment of neutrophils to endothelial cells: (A) neutrophils incubated with extracts in the concentration range 25–100 μg/mL; (B) endothelial cells incubated with extracts in the concentration range 25–100 μg/mL; (C) endothelial cells incubated with extracts in the concentration range 12.5–50 μg/mL and neutrophils incubated with extracts in the concentration range 12.5–50 μg/mL; (D) intercellular adhesion molecule-1 (ICAM-1) expression on the surface of stimulated endothelial cells [%]; (E) E-selectin expression on the surface of stimulated endothelial cells [%]; Percent of cells with diminished membrane integrity (propidium iodide positive cells IP (+)): (F) neutrophils; (G) HUVECs. Data are expressed as the mean ± SEM; at least three independent experiments were performed, and they were assayed in duplicate. Experiments were performed using cells from different donors. Statistical significance #P < 0.01 compared to the non-stimulated control; *P < 0.05, **P < 0.01, ***P < 0.001 decrease compared to stimulated control (ST/ LPS/ TNF).