Figure 3.

Tau seeding activity precedes tau pathology along the Braak Tau Pathway. (A) Lysates along the Braak Tau Pathway for cases ranging from Braak I to Braak VI were all run on the tau seeding assay. Tau seeding values across the all brain regions analyzed were aggregated into a single value for each case and plotted against the Braak status of that brain. A significant direct correlation emerged, whereby as the Braak stage of the brain increases, so does the tau seeding activity across the whole brain. Spearman correlation. (B) Heatmap of tau seeding across the brain regions in the Braak Tau Pathway in human brains ranging from Braak I to Braak VI. Regions are organized left to right according to the progression of tau pathology throughout the course of AD; the EC is the first region to be impacted by tau pathology and the V1 is the last region. (C) Plots broken up based on the Braak stage of the brains. When compared to the unaffected cerebellum, tau seeding can be detected at least one region past where tau pathology is detected. Kruskal-Wallis test, Dunn's post-hoc multiple comparisons. #p < 0.01, *p < 0.05, **p < 0.01. Individual dots represent individual human cases. Mean ± SEM.