Table 1:
Whole genome bisulfite sequencing of sperm cells (Sperm) and somatic cells from brain prefrontal cortex (CORTEX), mammary gland (MAM), and blood (WBC) of dairy cattle
Sample | Mapped reads | Bisulfite conversion rate (%) | Methylation level (%) | Average CpG coverage (× fold) | Whole genome CpG covered (%) |
---|---|---|---|---|---|
CORTEX1 | 407,336,580 | 99.31–99.44 | 74.50 | 7.03 | 95 |
CORTEX2 | 437,071,564 | 99.34–99.47 | 76.30 | 6.39 | 86 |
MAM1 | 344,055,380 | 99.38–99.48 | 73.10 | 6.19 | 94 |
MAM2 | 393,720,380 | 99.38–99.44 | 72.80 | 7.18 | 96 |
WBC1 | 280,887,870 | 99.40–99.46 | 78.10 | 6.41 | 94 |
WBC2 | 282,543,428 | 99.25–99.44 | 77.50 | 6.83 | 93 |
Sperm1 A | 296,241,574 | 99.43–99.52 | 74.00 | 5.75 | 95 |
Sperm1 B | 302,185,176 | 99.43–99.51 | 75.70 | 5.49 | 93 |
Sperm2 A | 368,996,008 | 99.40–99.51 | 76.40 | 7.10 | 96 |
Sperm2 B | 289,192,004 | 99.34–99.41 | 75.70 | 5.75 | 94 |