TABLE 1.
Primer sequences used for real-time PCR
| Genes | Primer Sequence (5′-3′) | |
| Forward | Reverse | |
| ACAT1 | TCGCACTCCTCATCCTATTCG | GTACCAGCCTTCCTTCATCGAT |
| ACAT2 | AGACTTGGTGCAATGGACTCGAC | CATAGGGCCCGATCCAACAG |
| UCP1 | TCATCATCAATTGTACAGAGCTGGTA | CGTCATCTGCCAGTATTTTGTTG |
| Leptin | CACACACGCAGTCGGTATCC | AGCCCAGGAATGAAGTCCAA |
| SREBP1 | GGCACTGAAGCAAAGCTGAAT | GCAAGAAGCGGATGTAGTCGAT |
| PPARr | CCCAATGGTTGCTGATTACAAAT | CTACTTTGATCGCACTTTGGTATTCT |
| FAS | ACCACTGCATTGACGGCCGG | GGGTCAGGCGGGAGACCGAT |
| SCD1 | GGTGATGTTCCAGAGGAGGTACTAC | AGCGTGGGCAGGATGAAG |
| MGAT1 | CTGGTTCTGTTTCCCGTTGT | TGGGTCAAGGCCATCTTAAC |
| DGAT1 | GTGCACAAGTGGTGCATCAG | CAGTGGGATCTGAGCCATCA |
| DGAT2 | GCCGTGTGGCGCTACTTC | GTGGTCAGCAGGTTGTGTGTCT |
| Adipoq | GGAACTTGTGCAGGTTGGAT | GCTTCTCCAGGCTCTCCTTT |
| Resistin | TGCCAGTGTGCAAGGATAGACT | CGCTCACTTCCCCGACAT |
| SR-BI | GGCTGCTGTTTGCTGCG | GCTGCTTGATGAGGGAGGG |
| CD36 | GTTCTTCCAGCCAATGCCTTT | ATGTCTAGCACACCATAAGATGTACAGTT |
| ABCA1 | GGTTTGGAGATGGTTATACAATAGTTGT | TTCCCGGAAACGCAAGTC |
| ABCG1 | AGGTCTCAGCCTTCTAAAGTTCCTC | TCTCTCGAAGTGAATGAAATTTATCG |
| SREBP1a | AGATGTGCGAACTGGACACA | CATCTTTAAAGCAGCGGGTG |
| SREBP1c | GGAGCCATGGATTGCACATT | CATCTTTAAAGCAGCGGGTG |
| SREBP2 | TGTGAACCTGGCCGAGTGT | CGCTGTCAGGTGGATCTCAA |
| β-Actin | AGATGACCCAGATCATGTTTGAGA | CACAGCCTGGATGGCTACGT |
| RPL27 | CCTGGCCGGACGCTACT | AGGTGCCATCGTCAATGTTCT |
UCP1, uncoupling protein 1.