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. 2018 Mar 20;59(5):854–863. doi: 10.1194/jlr.M083618

Fig. 6.

Fig. 6.

Induced AIBP expression in zebrafish reduces diet-induced vascular lipid accumulation. A: Construct used to make transgenic hsp70:apoa1bp-2A-mRFP zebrafish. B: Heat shock-induced Apoa1bp protein expression. Embryos were subjected for 1 h to heat shock (37°C) at 1 dpf and again at 2 dpf. Three mRFP-negative and three mRFP-positive embryos were selected and homogenized in SDS-PAGE loading buffer. Lysates were run on SDS-PAGE, blotted, and probed with anti-zebrafish Apoa1bp and tubulin antibodies. C: Vascular lipid accumulation. Transgenic hsp70:apoa1bp-2A-mRFP zebrafish were fed control or 4% cholesterol diet supplemented with TopFluor-cholesterol for 10 days, and one group was subjected to three heat shock sessions during the 10-day feeding period. Left demonstrates heat shock-induced expression of mRFP (red) in zebrafish. Right shows vascular cholesterol deposits (green). White dashed lines trace the caudal vein in zebrafish. D: Quantification of vascular lipid deposits. Mean ± SEM; n = 4–11; * P < 0.05 (one-way ANOVA with Tukey’s multiple comparison test).