Fig. 6.

Structurally distinct P2X7 antagonists do not induce ABCA1 or apoE. CCF-STTG1 cells were treated with 10 μM of published P2X7 antagonists A740003, A804598, A839977, BBG, JNJ47965567, A438079, AZ11645373, AZ10606120, and AZD9056, along with AZ-1, AZ-2, and controls for 72 h. A: Representative immunoblots of cellular ABCA1. B: Quantification of ABCA1 protein levels. The graphs represent fold changes over DMSO control (dashed line) and ±95% confidence interval from three independent experiments. *P < 0.05, ***P < 0.001 compared with vehicle control using blocked two-way ANOVA post hoc tests. C: Chemical structures of the tested P2X7 antagonists.