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. 2018 Mar 21;59(5):830–842. doi: 10.1194/jlr.M081851

Fig. 7.

Fig. 7.

Induction of ABCA1 by AZ-1 and AZ-2 is P2X7 receptor independent. A: Representative immunoblot confirming lack of P2X7 receptor protein in primary mixed glia derived from P2rx7−/− mice. Untreated cell lysates of each genotype from three experiments are presented. ABCA1 mRNA levels (B) and cellular ABCA1 protein levels(C) in WT mixed glia treated with DMSO, 1 μM T0901317, or 10 μM AZ-1 or AZ-2 for 72 h. ABCA1 mRNA levels (D) and cellular ABCA1 protein levels (E) in P2rx7−/− mixed glia treated with DMSO, 1 μM T0901317, or 10 μM AZ-1 or AZ-2 for 72 h. Graphs represent fold change over DMSO control (dashed line) and ±95% confidence interval from N independent experiments indicated in brackets. **P < 0.01, ***P < 0.001 compared with vehicle control by blocked two-way ANOVA post hoc tests. F: Representative immunoblot showing cellular P2X7 protein levels in WT and P2RX7−/− CCF-STTG1 (CCF) cells. G: ABCA1 mRNA levels in WT CCF-STTG1 (WT), P2RX7−/− clone 1 (P2RX7−/− cl1), P2RX7−/− clone 2 (P2RX7−/− cl2), and P2RX7−/− clone 3 (P2RX7−/− cl3) CCF-STTG1 cells treated with DMSO, 1 μM T0901317, or 10 μM AZ-1 or AZ-2 for 72 h. The graphs represent fold changes over respective DMSO control (dashed line) and ±95% confidence interval from four experiments for WT and three experiments for each P2RX7−/− clone. *P < 0.05, **P < 0.01, ***P < 0.001 compared with respective DMSO by blocked two-way ANOVA post hoc tests. ns, not significant.