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. 2018 Apr 24;11:134. doi: 10.3389/fnmol.2018.00134

FIGURE 1.

FIGURE 1

Correlation of neural markers with heparan sulfate proteoglycan (HSPG) core proteins throughout extended human mesenchymal stem cell (hMSC) in vitro expansion. (A) hMSC populations were expanded for >80 population doublings in vitro and gene expression of key HSPG core proteins correlated with key neural markers throughout different phases of growth (Phase A-C). (B–D) Representative immunocytochemistry (ICC) images of neural stemness and lineage markers simultaneously expressed with key HSPG core proteins in undifferentiated hMSCs. Primary antibodies against each protein were used. Secondary antibodies: FITC: green; Cy3: yellow; and AF594: red. All cultures were counterstained with DAPI to identify nuclei. Scale bars represent 70 μm. (E–G) Q-PCR gene expression of (E) specific glial lineage markers: GFAP, GALC and OLIG2 (F) HSPG core proteins: SDC1, SDC4, and GPC4 (G) HSPG core protein GPC1, with neural stemness (Nestin) and glial (CD44) markers.