Figure 2.

Method validation for defining ER selectivity based on (A) ERα-dependent alkaline phosphatase induction; (B) ERβ-ERE-luciferase induction using E₂ (black, open circles), PPT (ERα-selective ligand) (red, filled diamonds), 8-prenylnaringenin (2) (pink, filled squares), DPN (ERβ selective ligand) (green, open squares), and genistein (1) (ERβ selective ligand) (purple, filled triangles). The methods for the Ishikawa and ERE-luciferase assays are described in the Experimental Section. The data represent the means ± SEM of three independent determinations.