Figure 6.
Induction of differential estrogenic activity with 8-prenylapigenin (3) (blue, filled circles) compared to E2 (black, open circles) and the known phytoestrogens genistein (1) (purple, filled triangles) and 8-prenylnaringenin (2) (pink, filled squares) in (A) an ERα-dependent alkaline phosphatase activity induction assay in Ishikawa cells and (B) an ERβ-ERE-luciferase assay in MDA-MB-231/β41 cells. The methods for the Ishikawa and ERE-luciferase assays are described in the Experimental Section. The data represent the means ± SEM of three independent determinations.