CFM images and flow cytometry analysis of different types
of cells
after incubation with 3 at the final concentration of
20 μM at 37 °C for different periods. (A) Flow cytometry
analysis of 3 in ID-8 at 0 h (red, control), 1 h (green),
and 3 h (blue). (B, C) CFM images and flow cytometry analysis in ID8
at 1 and 3 h, respectively. (D) Flow cytometry analysis of 3 in MX-1 at 0 h (red, control), 1 h (green), and 3 h (blue). (E,
F) CFM images and flow cytometry analysis in MX-1 at 1 and 3 h, respectively.
(For full internalization study results using ID8 and MX-1 cancer
cells, as well as WI-38 normal human lung fibroblast cells, see Figure S49 in the Supporting Information.).