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. 1999 Jun;120(2):473–480. doi: 10.1104/pp.120.2.473

Figure 2.

Figure 2

Northern analysis of Alfin1 and MsPRP2 expression in control and transgenic calli from Alfin1 sense transformants. Lanes 1 and 2, RNA isolated from untransformed NaCl-tolerant callus grown with or without 171 mm NaCl for 4 weeks; lane 3, RNA isolated from untransformed NaCl-sensitive callus; lane 4, RNA isolated from NaCl-sensitive callus transformed with pGA vector (1V); lanes 5 to 9, RNA isolated from NaCl-sensitive callus transformed with Alfin1 sense construct (S1, S2, S4, and S6 are independently transformed lines); and lane 9, RNA isolated from S2-transformed callus grown in 171 mm NaCl. Each lane contained 10 μg of total RNA. Each blot was hybridized sequentially with the following probes: Alfin1, the large EcoRI fragment (Fig. 1); MsPRP2, the carboxy-terminal and 3′-untranslated region fragment (Winicov and Deutch, 1994); and Msc27, the fragment of a constitutively expressed alfalfa gene.