Figure 3.

Northern analysis of Alfin1 expression in control and transgenic plants from Alfin1 sense transformants. RNA was isolated from leaves of control and transgenic plants. Lane Con, No. 1 control NaCl-sensitive parent plant for all transformations; lanes S1, S2, and S3, plants transformed with the Alfin1 sense construct and regenerated from transformed callus; and lane V, vector-transformed plant. Each blot was hybridized sequentially with the following probes: Alfin1, large EcoRI fragment (Fig. 1); pGA-vector, EcoRI/BglII fragment from pGA643 to show readthrough of the Alfin1 transgene; and Msc27, fragment of a constitutively expressed alfalfa gene. Each lane contained 10 μg of total RNA.