Table II.
Growth properties of Alfin1-sense-transformed plants on 128 mm NaCl
Plant | Survival | New Leaf Growth | Percentage |
---|---|---|---|
g/plant | |||
No. 1 (parent) | 4 /5 | 0.56 ± 0.32 | 100 |
No. 1 + vector | 3 /4 | 0.42 ± 0.32 | 75 |
No. 1 + sense-1a | 7 /7 | 1.40 ± 0.17 | 250 |
No. 1 + sense-2a | 7 /7 | 1.85 ± 0.23 | 330 |
No. 1 + sense-3a | 3 /3 | 1.45 ± 0.32 | 259 |
IW9b | 7 /7 | 1.10 ± 0.18 | 196 |
Multiple rooted cuttings from each plant were established in individual Conetainers in perlite for 6 weeks and grown on one-quarter-strength Hoagland solution. All shoots were then cut back to the crown. Growth was continued from that point on one-quarter-strength Hoagland solution supplemented with 128 mm (0.75%) NaCl. The newly regrown shoots were harvested and weighed after 17 d. Data are means ± sd.
Plants sense-1, sense-2, and sense-3 correspond to S1, S2, and S3 shown in Figure 4.
NaCl-tolerant plant regenerated after selection in tissue culture from parent plant no. 1 (Winicov, 1991).