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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Biotechnol Bioeng. 2014 Jul 4;111(11):2239–2251. doi: 10.1002/bit.25282

Figure 4.

Figure 4

MSC PGE2 secretion promotes macrophage reprogramming. A: Supernatant from M1: MSC co-cultures treated with LPS (1 μg/mL) were assessed for PGE2 concentration. M1 macrophages in basal and stimulated conditions did not produce PGE2. However, in the presence of MSC ~6.5 ng/mL was detected. When a COX2 inhibitor, indomethacin (10 μM), is administered to the co-culture MSC PGE2 secretion is eliminated. *Represents statistical significance (P < 0.01) relative to LPS, medium and indomethacin. B: CD206 expression and (C) TNF-α secretion were assessed with indomethacin present in the MSC:M1 co-culture. The inhibition of MSC PGE2 secretion prevented the percent increase of CD206 expressing cells as well as the attenuation of TNF-α. *Represents statistical significance (P < 0.01) between MSC:M1 conditions in the presence and absence of indomethacin.