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Imaging phantom for fluorescence anisotropy imaging. (a–f) Fluorescence intensity (a–c) and fluorescence anisotropy (d–f) images of two separate (a,b,d,e) and mixed (c,f) bead populations with different relative intensity concentrations. At a high fluorophore concentration (B beads), homo-FRET takes place, reducing the effective value of fluorescence anisotropy69. The two bead populations can then be resolved on the basis of their fluorescence anisotropy values. Scale bars, 17 µm.
