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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Nat Protoc. 2017 Jun 29;12(7):1472–1497. doi: 10.1038/nprot.2017.043

Figure 7.

Figure 7

Optical characterization of three calibration solutions, ruthenium tris(bipyridyl) [Ru(bipy)3] in PBS (Ru in PBS), fluorescein in PBS (FL in PBS), pyridine 1 in propylene glycol (Py1 in PG). (a–f) One-photon excitation spectra (a–c) and one-photon fluorescence anisotropy emission (d–f) as a function of excitation wavelength for emissions at 600 nm (a,d), 525 nm (b,e), and 680 nm (c,f). (g–i) Two-photon fluorescence anisotropy as a function of wavelength for the corresponding dyes, for detection at 600 nm (g,h) and 517 nm (i).