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. 2018 Apr 24;9:531. doi: 10.3389/fpls.2018.00531

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Copyright © 2018 Dechorgnat, Francis, Dhugga, Rafalski, Tyerman and Kaiser.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inorganic nitrogen content and enzymatic activities in roots of B73 (white) and F44 (black) maize inbreds. Measurements of nitrate (A), nitrite (B), and ammonium content (C). Values are means (±SE) from 4 to 8 individual plants. Similar results were obtained in two other independent experiments. Analysis of nitrate reductase activity (D), nitrite reductase activity (E), and asparagine synthetase activity (F). Values are means (±SE) from four individual plants. Similar results were obtained in another independent experiment. (G) Measurements of glutamine synthetase activity in B73 (open circles) and F44 (closed circles) in eight individual plants from two independent experiments, histograms represent the mean values (±SE). Asterisks indicate significant differences between lines at ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.005 (Student’s t-test).